EEF2K antibody (pSer366)

Catalog No. ABIN1531227

Product Details anti-EEF2K Antibody

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Binding Specificity: AA 331-380, pSer366

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity:

eEF2K (Phospho-Ser366) Antibody detects endogenous levels of eEF2K only when phosphorylated at Ser366.

PhosphorylationH:S366 R:S365

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Comment:

Unigene-Number: Hs.633744 (NCBI Gene Symbol: EEF2K)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-EEF2K antibody (ABIN1531227)

Usui, Nijima, Sakatsume, Otani, Kameshima, Okada, Yamawaki: "Eukaryotic elongation factor2 kinase controls proliferation and migration of vascular smooth muscle cells." in: Acta physiologica (Oxford, England), (2014) (PubMed).

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: eEF2K (EEF2K Products)

Synonyms: HSU93850 antibody, eEF-2K antibody, C86191 antibody, SMEF2K antibody, eEF-2 kinase antibody, cb365 antibody, fa04b08 antibody, wu:fa04b08 antibody, zgc:100973 antibody, eukaryotic elongation factor 2 kinase antibody, eukaryotic elongation factor-2 kinase antibody, EEF2K antibody, Eef2k antibody, eef2k antibody, LOC101930123 antibody

Background:

Synonyms: Calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase, EF2K, Elongation factor 2 kinase, eEF-2 kinase, eEF-2K, kinase eEF2K

NCBI Gene Symbol: EEF2K

Molecular Weight: 82 kDa

Gene ID: 29904

OMIM: 606968

UniProt: O00418

Pathways: AMPK Signaling