EEF2 antibody (pThr56)

Catalog No. ABIN1531226

Product Details anti-EEF2 Antibody

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Binding Specificity: AA 31-80, pThr56

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity:

eEF2 (Phospho-Thr56) Antibody detects endogenous levels of eEF2 only when phosphorylated at Thr56.

PhosphorylationH:T56 M:T56 R:T56

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

Comment:

Unigene-Number: Hs.515070 (NCBI Gene Symbol: EEF2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: eEF2 (EEF2 Products)

Background:

Synonyms: EEF2, EF-2, EF2, Elongation factor 2

NCBI Gene Symbol: EEF2

Molecular Weight: 95 kDa

Gene ID: 1938

OMIM: 130610

UniProt: P13639

Pathways: AMPK Signaling