CROT antibody (pThr290)

Catalog No. ABIN1531220

Product Details anti-CROT Antibody

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Binding Specificity: AA 256-305, pThr290

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CROT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity:

COT (Phospho-Thr290) Antibody detects endogenous levels of COT only when phosphorylated at Thr290.

PhosphorylationH:T290 M:T290 R:T290

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Comment:

Unigene-Number: Hs.432453 (NCBI Gene Symbol: MAP3K8)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CROT

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Alternative Name: COT (CROT Products)

Synonyms: CROT antibody, cot antibody, zgc:110643 antibody, COT antibody, 1200003H03Rik antibody, carnitine O-octanoyltransferase antibody, CROT antibody, crot antibody, Crot antibody

Background:

Synonyms: C-COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, M3K8, MAP3K8, Mitogen-activated protein kinase kinase kinase 8, TPL-2, TPL2, Tpl2, Tumor progression locus 2, kinase Cot

NCBI Gene Symbol: MAP3K8

Molecular Weight: 52 kDa

Gene ID: 1326

OMIM: 191195

UniProt: P41279

Pathways: Monocarboxylic Acid Catabolic Process