Digoxigenin antibody (Alkaline Phosphatase (AP))
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- Target See all Digoxigenin (DIG) products
- Digoxigenin (DIG)
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Reactivity
- Chemical
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Digoxigenin antibody is conjugated to Alkaline Phosphatase (AP)
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), In situ hybridization (ISH), Southern Blotting (SB)
- Specificity
- Reacts with free and bound digoxigenin
- Purification
- Purified (protein A)
- Immunogen
- Digoxigenin
- Clone
- 21H8
- Isotype
- IgG1 kappa
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- Application Notes
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Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- IHC : 1/200 to 1/2000 - ELISA : 1/500 to 1/5000 - WB : 1/500 to 1/5000 - SB : 1/500 to 1/5000 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- Tris 10 mM NaCl 0.14M,MgCl2/ZnCl2 1 mM, Glycerol 50 % , Thiomersal 0.02 %
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Digoxigenin (DIG)
- Alternative Name
- Digoxigenin (DIG Products)
- Target Type
- Chemical
- Background
- Digoxigenin (DIG) is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications.
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