PKC theta antibody (AA 15-294)

Catalog No. ABIN1501904

Product Details anti-PKC theta Antibody

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Binding Specificity: AA 15-294

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human, mouse PRKCQ / PKC-Theta

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine (98%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 15 and 294 of PKC theta (SwissProt Q04759). Percent identity by BLAST analysis: Human (100%), Bovine (98%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Alternative Name: PRKCQ / PKC-Theta (PRKCQ Products)

Synonyms: PRKCT antibody, nPKC-theta antibody, A130035A12Rik antibody, AW494342 antibody, PKC-0 antibody, PKC-theta antibody, PKCtheta antibody, Pkcq antibody, protein kinase C theta antibody, protein kinase C eta antibody, protein kinase C, theta antibody, PRKCQ antibody, PRKCH antibody, Prkcq antibody

Background: Name/Gene ID: PRKCQ
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCQ, NPKC-theta, PKC theta, PRKCT, Protein kinase C theta type, Protein kinase c theta, PKCtheta, Protein kinase C, theta

Gene ID: 5588

UniProt: Q04759

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling