TRIM25 antibody (AA 243-491)

Catalog No. ABIN1501503

Product Details anti-TRIM25 Antibody

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Binding Specificity: AA 243-491

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human TRIM25

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 243 and 491 (Q14258) of TRIM25. Percent identity by BLAST analysis:

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25 (TRIM25 Products)

Synonyms: zgc:55773 antibody, MGC130751 antibody, EFP antibody, RNF147 antibody, Z147 antibody, ZNF147 antibody, AA960166 antibody, AL022677 antibody, Zfp147 antibody, Efp antibody, Znf147 antibody, E3 ubiquitin/ISG15 ligase TRIM25 antibody, tripartite motif containing 25 antibody, E3 ubiquitin/ISG15 ligase TRIM25-like L homeolog antibody, microRNA 3614 antibody, tripartite motif-containing 25 antibody, LOC100146440 antibody, trim25 antibody, LOC100490741.L antibody, TRIM25 antibody, MIR3614 antibody, Trim25 antibody

Background: Name/Gene ID: TRIM25
Family: Tripartite Motif

Synonyms: TRIM25, EFP, Zinc finger protein 147, Z147, Zinc finger protein-147, ZNF147, Tripartite motif containing 25, RING finger protein 147, RNF147, Tripartite motif-containing 25

Gene ID: 7706

UniProt: Q14258