NCK1 antibody (AA 53-333)

Catalog No. ABIN1499652

Product Details anti-NCK1 Antibody

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Binding Specificity: AA 53-333

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: Human NCK1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rat, Bovine (99%) Mouse (98%) Porcine (97%) Xenopus (84%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 53 and 333 of NCK1 (SwissProt P16333). Percent identity by BLAST analysis: Human (100%), Rat, Bovine (99%), Mouse (98%), Porcine (97%), Xenopus (84%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1 / NCK (NCK1 Products)

Synonyms: NCK antibody, NCKalpha antibody, nck-1 antibody, NCK1 antibody, 6330586M15Rik antibody, D230010O13Rik antibody, Nck antibody, Nck-1 antibody, Nck1 antibody, nck antibody, nck1-a antibody, nck1-b antibody, nckalpha antibody, NCK adaptor protein 1 antibody, non-catalytic region of tyrosine kinase adaptor protein 1 antibody, NCK adaptor protein 1b antibody, NCK adaptor protein 1 L homeolog antibody, NCK1 antibody, Nck1 antibody, nck1b antibody, nck1.L antibody

Background: Name/Gene ID: NCK1

Synonyms: NCK1, Cytoplasmic protein nck, Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, NCKalpha, Melanoma NCK protein, NCK, NCK tyrosine kinase

Gene ID: 4690

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals