Calpastatin antibody (AA 275-700)

Catalog No. ABIN1497106

Product Details anti-Calpastatin Antibody

Target: Calpastatin (CAST)

Binding Specificity: AA 275-700

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpastatin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human CAST / Calpastatin

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rabbit (80%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 275 and 700 of Calpastatin (SwissProt P20810). Percent identity by BLAST analysis: Human (100%), Rabbit (80%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for Calpastatin

Target: Calpastatin (CAST)

Alternative Name: CAST / Calpastatin (CAST Products)

Synonyms: BS-17 antibody, calp3 antibody, CAST antibody, sb:cb966 antibody, zgc:194249 antibody, cast antibody, DKFZp469E0716 antibody, calpastatin antibody, calpastatin S homeolog antibody, Calpastatin antibody, CAST antibody, Cast antibody, cast.S antibody, cast antibody, VDBG_09182 antibody, ical antibody

Background: Name/Gene ID: CAST

Synonyms: CAST, Calpastatin, BS-17, Calpain inhibitor, Sperm BS-17 component

Gene ID: 831

UniProt: P20810