GLUD1 antibody (AA 93-107)

Catalog No. ABIN1496046

Product Details anti-GLUD1 Antibody

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Binding Specificity: AA 93-107

Reactivity: Human, Mouse, Horse, Pig, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GLUD1. This antibody is expected to recognize both reported isoforms (NP_005262.1, NP_036216.2).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Panda, Horse, Pig (100%) Rat, Opossum, Pufferfish (93%) Bovine, Xenopus, Stickleback (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-ESEEQKRNRVRGILR from an internal region of human GLUD1 (NP_005262.1, NP_036216.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Panda, Horse, Pig (100%), Rat, Opossum, Pufferfish (93%), Bovine, Xenopus, Stickleback (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55  kDa band observed in Human Liver and in Mouse Liver lysates (calculated MW of 61.4  kDa according to Human NP_005262.1 and NP_036216.2 and of 61.3 according to Mouse NP_032159.1. Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: GLUD1/Glutamate Dehydrogenase (GLUD1 Products)

Background: Name/Gene ID: GLUD1

Synonyms: GLUD1, GDH1, GDH 1, Glutamate dehydrogenase 1, Glutamate dehydrogenase 2, GDH, GLUD

Gene ID: 2746

NCBI Accession: NP_005262, NP_036216

UniProt: P00367

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect