CoREST antibody (AA 109-293)

Catalog No. ABIN1490833

Product Details anti-CoREST Antibody

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Binding Specificity: AA 109-293

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CoREST antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Brand: IHC-plus™

Specificity: Recognizes human CoREST, Mr ~66kD. Species cross-reactivity: Mouse and rat.

Purification: Protein A purified

Immunogen: GST fusion protein corresponding to residues 109-293 of human CoREST.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Positive control: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for CoREST

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Alternative Name: RCOR1 / COREST (RCOR1 Products)

Synonyms: COREST antibody, RCOR antibody, 5730409O11 antibody, 6720480E22Rik antibody, AU042633 antibody, D12Wsu95e antibody, mKIAA0071 antibody, rcor1 antibody, xcoREST antibody, RCOR1 antibody, im:6904264 antibody, si:dkey-46n18.7 antibody, zgc:158607 antibody, RGD1305743 antibody, REST corepressor 1 antibody, REST corepressor 1 L homeolog antibody, RCOR1 antibody, Rcor1 antibody, rcor1.L antibody, rcor1 antibody

Background: Name/Gene ID: RCOR1

Synonyms: RCOR1, COREST, Protein CoREST, REST corepressor 1, RCOR, KIAA0071, REST corepressor

Gene ID: 23186

UniProt: Q9UKL0

Pathways: Regulation of Hormone Metabolic Process, Chromatin Binding