GFRA2 antibody

Catalog No. ABIN1450225

Product Details anti-GFRA2 Antibody

Target: GFRA2 (GDNF Family Receptor alpha 2 (GFRA2))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFRA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Glial cell line-derived neurotrophic factor (GDNF) andneurturin (NTN) are two structurally related, potent neurotrophic factors thatplay key roles in the control of neuron survival and differentiation. GFRa-2 isa member of the GDNF receptor family. It is a glycosylphosphatidyl-inositol(GPI)-linkedcell surface receptor for both GDNF and NTN, and mediates activation of the RETtyrosine kinase receptor. This encoded protein acts preferentially as areceptor for NRTN compared to its other family member, GDNF family receptoralpha 1. http://www.ncbi.nlm.nih.gov/gene/2675

Characteristics: Mono- and polyclonal antibodies to human growth factors and neural growth factor receptors.

Purification: GFRa-2 affinity purified

Isotype: IgY

Application Details

Application Notes: Western immunoblotting, recommended dilution 1:5 000to 1:10 000, ELISA: 1:128 000 to 160 000, IF 1:500 to1:1000. Polyclonal antibody working titer has to beestablished practically for each particular antigen and assay format

Comment:

Synonym: GDNF receptor beta, Neurturin receptor alpha, RETligand 2TGF-beta-related neurotrophic factor receptor 2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: Concentrated ammonium sulphate in PBS pH 7.4

Handling Advice: As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use

Storage: 4 °C

Storage Comment: This product is shipped in non-frozen liquid form in ambient conditions

References for anti-GFRA2 antibody (ABIN1450225)

Siatra, Vatsellas, Chatzianastasiou, Balafas, Manolakou, Papapetropoulos, Agapaki, Mouchtouri, Ruchaya, Korovesi, Mavroidis, Thanos, Beis, Kokkinopoulos: "Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart." in: NPJ Regenerative medicine, Vol. 8, Issue 1, pp. 13, (2023) (PubMed).

Target Details for GFRA2

Target: GFRA2 (GDNF Family Receptor alpha 2 (GFRA2))

Alternative Name: GFRa-2 (GFRA2 Products)

Synonyms: GDNFRB antibody, NRTNR-ALPHA antibody, NTNRA antibody, RETL2 antibody, TRNR2 antibody, Retl2 antibody, NTNRALPHA antibody, ntnra antibody, retl2 antibody, trnr2 antibody, gdnfrb antibody, nrtnr-alpha antibody, gfra2 antibody, GDNF family receptor alpha 2 antibody, glial cell line derived neurotrophic factor family receptor alpha 2 antibody, GDNF family receptor alpha 2a antibody, GFRA2 antibody, Gfra2 antibody, gfra2 antibody, gfra2a antibody

Background: Recombinant His-tagged human GFRα-2 protein producedusing CHO-based cell line. For production of hGFRα-2, glycosylphosphatidyl-inositol GPI-anchor was removedand protein was secreted to the cell culture supernatant. Protein was purifiedby Ni-affinity chromatography following gel-filtration from cell culturesupernatant

UniProt: O00451