GFRA1 antibody

Catalog No. ABIN1450224

Product Details anti-GFRA1 Antibody

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFRA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Glial cell line-derived neurotrophic factor (GDNF) andneurturin (NTN) are two structurally related, potent neurotrophic factors thatplay key roles in the control of neuron survival and differentiation. The GFRa-1is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediatesactivation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674)

Characteristics: Mono- and polyclonal antibodies to human growth factors and neural growth factor receptors.

Purification: GFRa-1 affinity purified

Isotype: IgY

Application Details

Application Notes: Westernimmunoblotting, recommended dilution 1:5 000 to 1:10 000, ELISA: 1:80 000 to160 000,IF 1:1000 to 1:2000. Polyclonal antibody working titer has to be established practically for each particularantigen and assay format

Comment:

Synonym: RET ligand 1, TGF-beta related neurotrophic factorreceptor 1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: Concentrated ammonium sulphate in PBS pH 7.4

Handling Advice: As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use

Storage: 4 °C

Storage Comment: This product is shipped in non-frozen liquid form in ambient conditions

Target Details for GFRA1

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Alternative Name: GFRa-1 (GFRA1 Products)

Synonyms: gdnfr antibody, ret1l antibody, retl1 antibody, trnr1 antibody, gdnfra antibody, MGC122616 antibody, gfr-alpha-1 antibody, GDNFR antibody, GDNFRA antibody, GFR-ALPHA-1 antibody, RET1L antibody, RETL1 antibody, TRNR1 antibody, GDNFRALPHA antibody, GFRA1 antibody, AU042498 antibody, gfralpha1a antibody, zgc:110129 antibody, GDNF family receptor alpha 1 antibody, glial cell line derived neurotrophic factor family receptor alpha 1 antibody, gdnf family receptor alpha 1a antibody, gfra1 antibody, GFRA1 antibody, Gfra1 antibody, gfra1a antibody

Background: Recombinant His-tagged human GFRa-1 protein producedusing CHO-based cell line. For production of hGFRa-1, glycosylphosphatidyl-inositol GPI-anchor was removedand protein was secreted to the cell culture supernatant. Protein was purifiedby Ni-affinity chromatography following gel-filtration from cell culturesupernatant

UniProt: P56159