DPAGT1 antibody (N-Term)

Catalog No. ABIN1450055

Product Details anti-DPAGT1 Antibody

Target: DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPAGT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography purified via peptide column

Immunogen: 17 amino acid synthetic peptide near the amino terminus of Human DPAGT1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-DPAGT1 antibody (ABIN1450055)

Niewiadomska, Janik, Perlińska-Lenart, Piłsyk, Palamarczyk, Kruszewska: "The role of Alg13 N-acetylglucosaminyl transferase in the expression of pathogenic features of Candida albicans." in: Biochimica et biophysica acta. General subjects, Vol. 1861, Issue 4, pp. 789-801, (2017) (PubMed).

Target Details for DPAGT1

Target: DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))

Alternative Name: DPAGT1 (DPAGT1 Products)

Synonyms: AU021132 antibody, Dpagt2 antibody, GPT antibody, Gnpta antibody, DPAGT2 antibody, G1PT antibody, GNPTA antibody, GTR2 antibody, ALG7 antibody, CDG-Ij antibody, CDG1J antibody, CMSTA2 antibody, D11S366 antibody, DGPT antibody, DPAGT antibody, UAGT antibody, UGAT antibody, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) antibody, dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 antibody, Dpagt1 antibody, DPAGT1 antibody

Background: The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.Synonyms: DPAGT2, G1PT, GPT, GlcNAc-1-P transferase, N-acetylglucosamine-1-phosphate transferase, UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene ID: 1798

NCBI Accession: NP_001373