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GAPDH antibody (C-Term)

GAPDH Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Monkey, Cow, Dog, Sheep, Hamster, Horse, Goat, Guinea Pig, Cat, Fish, Avian, Donkey WB, IF, IHC (p), IHC (fro) Host: Goat Polyclonal unconjugated
Catalog No. ABIN1440016
  • Target See all GAPDH Antibodies
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    Binding Specificity
    • 33
    • 22
    • 13
    • 11
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 212
    • 144
    • 137
    • 69
    • 63
    • 51
    • 35
    • 26
    • 21
    • 19
    • 19
    • 18
    • 17
    • 16
    • 11
    • 10
    • 10
    • 8
    • 8
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Rabbit, Pig, Chicken, Monkey, Cow, Dog, Sheep, Hamster, Horse, Goat, Guinea Pig, Cat, Fish, Avian, Donkey
    Host
    • 160
    • 94
    • 16
    • 11
    • 4
    Goat
    Clonality
    • 186
    • 97
    Polyclonal
    Conjugate
    • 153
    • 33
    • 18
    • 17
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This GAPDH antibody is un-conjugated
    Application
    • 228
    • 96
    • 70
    • 65
    • 43
    • 37
    • 30
    • 24
    • 16
    • 16
    • 15
    • 13
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.
    Characteristics
    Glyceraldehyde-3-phosphate dehydrogenase
    Purification
    epitope affinity purified
    Immunogen
    Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
    Isotype
    IgG
    Top Product
    Discover our top product GAPDH Primary Antibody
  • Restrictions
    For Research Use only
  • Concentration
    2 mg/mL
    Buffer
    Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide
    Handling Advice
    The antibody solution should be gently mixed before use.
    Storage
    4 °C,-20 °C
    Storage Comment
    For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.
  • Ferreira, Rosa Soares, Ramalho, Ribeiro-Rodrigues, Máximo, Zuzarte, Girão, Pereira: "Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis." in: PLoS ONE, Vol. 14, Issue 10, pp. e0223790, (2020) (PubMed).

    Sanzà, Evans, Briggs, Cantero, Montoliu, Patel, Sviderskaya, Itzen, Figueiredo, Seabra, Hume: "Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a." in: Journal of cell science, Vol. 132, Issue 9, (2020) (PubMed).

    Aires, Boia, Rodrigues-Neves, Madeira, Marques, Ambrósio, Santiago: "Blockade of microglial adenosine A2A receptor suppresses elevated pressure-induced inflammation, oxidative stress, and cell death in retinal cells." in: Glia, Vol. 67, Issue 5, pp. 896-914, (2019) (PubMed).

    Robinson, Evans, Briggs, Ramalho, Hume: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).

    Ramalho, Toscano, Pereira, Girão, Gonçalves, Marques: "Hyperglycemia-induced degradation of HIF-1α contributes to impaired response of cardiomyocytes to hypoxia." in: Revista portuguesa de cardiologia : orgão oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology, Vol. 36, Issue 5, pp. 367-373, (2018) (PubMed).

    Ferreira, Soares, Ramalho, Pereira, Girao: "K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy." in: Scientific reports, Vol. 5, pp. 10210, (2016) (PubMed).

    Casalou, Seixas, Portelinha, Pintado, Barros, Ramalho, Lopes, Barral: "Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration." in: Journal of cell science, Vol. 127, Issue Pt 12, pp. 2709-22, (2015) (PubMed).

    Ribeiro-Rodrigues, Catarino, Marques, Ferreira, Martins-Marques, Pereira, Girão: "AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 11, pp. 4629-41, (2015) (PubMed).

  • Target
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    Alternative Name
    GAPDH (GAPDH Products)
    Synonyms
    G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody
    Background
    GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
    Synonyms: glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.
    Ensembl identifier: ENSG00000111640
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