Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.
Characteristics
Glyceraldehyde-3-phosphate dehydrogenase
Purification
epitope affinity purified
Immunogen
Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
GAPDH
Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Cow, Various Species
WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide
Handling Advice
The antibody solution should be gently mixed before use.
Storage
4 °C,-20 °C
Storage Comment
For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.
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GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Synonyms: glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody. Ensembl identifier: ENSG00000111640