GAPDH antibody (C-Term)

Catalog No. ABIN1440016

Product Details anti-GAPDH Antibody

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Dog, Monkey, Sheep, Cow, Hamster, Cat, Horse, Guinea Pig, Goat, Fish, Avian, Donkey

Host: Goat

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Characteristics: Glyceraldehyde-3-phosphate dehydrogenase

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-GAPDH antibody (ABIN1440016)

Ferreira, Rosa Soares, Ramalho, Ribeiro-Rodrigues, Máximo, Zuzarte, Girão, Pereira: "Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis." in: PLoS ONE, Vol. 14, Issue 10, pp. e0223790, (2020) (PubMed).

Sanzà, Evans, Briggs, Cantero, Montoliu, Patel, Sviderskaya, Itzen, Figueiredo, Seabra, Hume: "Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a." in: Journal of cell science, Vol. 132, Issue 9, (2020) (PubMed).

Aires, Boia, Rodrigues-Neves, Madeira, Marques, Ambrósio, Santiago: "Blockade of microglial adenosine A2A receptor suppresses elevated pressure-induced inflammation, oxidative stress, and cell death in retinal cells." in: Glia, Vol. 67, Issue 5, pp. 896-914, (2019) (PubMed).

Robinson, Evans, Briggs, Ramalho, Hume: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).

Ramalho, Toscano, Pereira, Girão, Gonçalves, Marques: "Hyperglycemia-induced degradation of HIF-1α contributes to impaired response of cardiomyocytes to hypoxia." in: Revista portuguesa de cardiologia : orgão oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology, Vol. 36, Issue 5, pp. 367-373, (2018) (PubMed).

Ferreira, Soares, Ramalho, Pereira, Girao: "K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy." in: Scientific reports, Vol. 5, pp. 10210, (2016) (PubMed).

Casalou, Seixas, Portelinha, Pintado, Barros, Ramalho, Lopes, Barral: "Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration." in: Journal of cell science, Vol. 127, Issue Pt 12, pp. 2709-22, (2015) (PubMed).

Ribeiro-Rodrigues, Catarino, Marques, Ferreira, Martins-Marques, Pereira, Girão: "AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 11, pp. 4629-41, (2015) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH (GAPDH Products)

Background: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Synonyms: glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.
Ensembl identifier: ENSG00000111640