UIS4 antibody

Catalog No. ABIN1440010

Product Details

Target: UIS4

Reactivity: Plasmodium

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF)

Specificity: Detects UIS4 in Plasmodium berghei

Characteristics: up-regulated in infective sporozoites gene 4

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References

Sanz, Aquilini, Tweedell, Verma, Hamerly, Hritzo, Tripathi, Machado, Churcher, Rodrigues, Izquierdo, Dinglasan: "Protein O-Fucosyltransferase 2 Is Not Essential for Plasmodium berghei Development." in: Frontiers in cellular and infection microbiology, Vol. 9, pp. 238, (2020) (PubMed).

Zuzarte-Luís, Mello-Vieira, Marreiros, Liehl, Chora, Carret, Carvalho, Mota: "Dietary alterations modulate susceptibility to Plasmodium infection." in: Nature microbiology, Vol. 2, Issue 12, pp. 1600-1607, (2018) (PubMed).

Santos, Egarter, Zuzarte-Luís, Kumar, Moreau, Kehrer, Pinto, Costa, Franke-Fayard, Janse, Frischknecht, Mair: "Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts." in: eLife, Vol. 6, (2018) (PubMed).

Manzoni, Marinach, Topçu, Briquet, Grand, Tolle, Gransagne, Lescar, Andolina, Franetich, Zeisel, Huby, Rubinstein, Snounou, Mazier, Nosten, Baumert, Silvie: "Plasmodium P36 determines host cell receptor usage during sporozoite invasion." in: eLife, Vol. 6, (2018) (PubMed).

Real, Rodrigues, Cabal, Enguita, Mancio-Silva, Mello-Vieira, Beatty, Vera, Zuzarte-Luís, Figueira, Mair, Mota: "Plasmodium UIS3 sequesters host LC3 to avoid elimination by autophagy in hepatocytes." in: Nature microbiology, Vol. 3, Issue 1, pp. 17-25, (2018) (PubMed).

Silva, Guerreiro, Santos, Braks, Janse, Mair: "Translational Control of UIS4 Protein of the Host-Parasite Interface Is Mediated by the RNA Binding Protein Puf2 in Plasmodium berghei Sporozoites." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147940, (2016) (PubMed).

Risco-Castillo, Topçu, Marinach, Manzoni, Bigorgne, Briquet, Baudin, Lebrun, Dubremetz, Silvie: "Malaria Sporozoites Traverse Host Cells within Transient Vacuoles." in: Cell host & microbe, Vol. 18, Issue 5, pp. 593-603, (2016) (PubMed).

Thieleke-Matos, Lopes da Silva, Cabrita-Santos, Portal, Rodrigues, Zuzarte-Luis, Ramalho, Futter, Mota, Barral, Seabra: "Host cell autophagy contributes to Plasmodium liver development." in: Cellular microbiology, Vol. 18, Issue 3, pp. 437-50, (2016) (PubMed).

Thieleke-Matos, da Silva, Cabrita-Santos, Pires, Ramalho, Ikonomov, Seixas, Shisheva, Seabra, Barral: "Host PI(3,5)P2 activity is required for Plasmodium berghei growth during liver stage infection." in: Traffic (Copenhagen, Denmark), Vol. 15, Issue 10, pp. 1066-82, (2015) (PubMed).

Target Details

Target: UIS4

Background: UIS4 protein is expressed throughout liver stage development and localizes to the parasite vacuole (parasitophorous vacuole membrane).