RAB27A antibody (C-Term)

Catalog No. ABIN1440000

Product Details anti-RAB27A Antibody

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Goat, Horse, Sheep, Dog, Guinea Pig, Rabbit, Monkey, Chicken, Pig, Cat, Donkey, Hamster, Avian

Host: Goat

Clonality: Polyclonal

Conjugate: This RAB27A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a. This antibody also recognizes Rab27b protein.

Characteristics: RAB27A, member RAS oncogene family

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a produced in E. coli.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Polyclonal antibody supplied as a 200 µL (1 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-RAB27A antibody (ABIN1440000)

Alzahofi, Welz, Robinson, Page, Briggs, Stainthorp, Reekes, Elbe, Straub, Kallemeijn, Tate, Goff, Sviderskaya, Cantero, Montoliu, Nedelec, Miles, Bailly, Kerkhoff, Hume: "Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins." in: Nature communications, Vol. 11, Issue 1, pp. 3495, (2020) (PubMed).

Pelkonen, Reinisalo, Morin-Picardat, Kidron, Urtti: "Isolation of Intact and Functional Melanosomes from the Retinal Pigment Epithelium." in: PLoS ONE, Vol. 11, Issue 8, pp. e0160352, (2017) (PubMed).

Reichhart, Markowski, Ishiyama, Wagner, Crespo-Garcia, Schorb, Ramalho, Milenkovic, Föckler, Seabra, Strauß: "Rab27a GTPase modulates L-type Ca2+ channel function via interaction with the II-III linker of CaV1.3 subunit." in: Cellular signalling, Vol. 27, Issue 11, pp. 2231-40, (2016) (PubMed).

Target Details for RAB27A

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Alternative Name: Rab27a (RAB27A Products)

Synonyms: MGC80943 antibody, RAB27A antibody, gs2 antibody, ram antibody, rab27 antibody, MGC89827 antibody, hst18676 antibody, zgc:114135 antibody, GS2 antibody, HsT18676 antibody, RAB27 antibody, RAM antibody, 2210402C08Rik antibody, 2410003M20Rik antibody, 4933437C11Rik antibody, ash antibody, RAB27A, member RAS oncogene family L homeolog antibody, RAB27A, member RAS oncogene family antibody, RAB27A, member RAS oncogene family S homeolog antibody, rab27a.L antibody, RAB27A antibody, rab27a antibody, rab27a.S antibody, Rab27a antibody

Background: Rab27a belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.
Synonyms: GS2, GTP-binding protein Ram, rab-27, ras-related protein Rab-27A, RAB27, RAM.
Ensembl identifier: ENSG00000069974

Pathways: Peptide Hormone Metabolism