GFP antibody

Catalog No. ABIN1439999

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea aequorea

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.

Characteristics: Green Fluorescent Protein

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-GFP antibody (ABIN1439999)

Panayiotou, Michael, Zaravinos, Demirag, Achilleos, Strati: "Human papillomavirus E7 binds Oct4 and regulates its activity in HPV-associated cervical cancers." in: PLoS pathogens, Vol. 16, Issue 4, pp. e1008468, (2020) (PubMed).

Wu, Yi, Manca, Javaid, Lauer, Glowatzki: "Sound exposure dynamically induces dopamine synthesis in cholinergic LOC efferents for feedback to auditory nerve fibers." in: eLife, Vol. 9, (2020) (PubMed).

Fukuda, Kaneshige, Kaji, Noguchi, Takemoto, Zhang, Tsujikawa, Kokubo, Uezumi, Maehara, Harada, Ohkawa, Fukada: "Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle." in: eLife, Vol. 8, (2020) (PubMed).

Zhang, Uezumi, Kaji, Tsujikawa, Andersen, Jensen, Fukada: "Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration." in: International journal of molecular sciences, Vol. 20, Issue 13, (2019) (PubMed).

Vyas, Wu, Jimenez, Glowatzki, Fuchs: "Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea." in: Scientific reports, Vol. 9, Issue 1, pp. 5549, (2019) (PubMed).

Larson, Mironova, Vanderpool, Waisman, Rash, Agarwal, Bergles: "Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility." in: eLife, Vol. 7, (2019) (PubMed).

Ramos-Nascimento, Kellen, Ferreira, Alenquer, Vale-Costa, Raposo, Delevoye, Amorim: "KIF13A mediates trafficking of influenza A virus ribonucleoproteins." in: Journal of cell science, Vol. 130, Issue 23, pp. 4038-4050, (2018) (PubMed).

Wu, Vyas, Glowatzki, Fuchs: "Opposing expression gradients of calcitonin-related polypeptide alpha (Calca/Cgrpα) and tyrosine hydroxylase (Th) in type II afferent neurons of the mouse cochlea." in: The Journal of comparative neurology, Vol. 526, Issue 3, pp. 425-438, (2018) (PubMed).

Festa, Chen, Berquez, Debaix, Tokonami, Prange, Hoek, Alessio, Raimondi, Nevo, Giles, Devuyst, Luciani: "Impaired autophagy bridges lysosomal storage disease and epithelial dysfunction in the kidney." in: Nature communications, Vol. 9, Issue 1, pp. 161, (2018) (PubMed).

Mimura, Takagi, Clever, Imamoto: "ELYS regulates the localization of LBR by modulating its phosphorylation state." in: Journal of cell science, Vol. 129, Issue 22, pp. 4200-4212, (2017) (PubMed).

Vale-Costa, Alenquer, Sousa, Kellen, Ramalho, Tranfield, Amorim: "Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors." in: Journal of cell science, Vol. 129, Issue 8, pp. 1697-710, (2017) (PubMed).

Agarwal, Wu, Hughes, Fukaya, Tischfield, Langseth, Wirtz, Bergles: "Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes." in: Neuron, Vol. 93, Issue 3, pp. 587-605.e7, (2017) (PubMed).

Vyas, Wu, Zimmerman, Fuchs, Glowatzki: "Tyrosine Hydroxylase Expression in Type II Cochlear Afferents in Mice." in: Journal of the Association for Research in Otolaryngology : JARO, Vol. 18, Issue 1, pp. 139-151, (2017) (PubMed).

Martins-Marques, Anjo, Pereira, Manadas, Girão: "Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 11, pp. 3040-55, (2016) (PubMed).

Sargiannidou, Kim, Kyriakoudi, Eun, Kleopa: "A start codon CMT1X mutation associated with transient encephalomyelitis causes complete loss of Cx32." in: Neurogenetics, Vol. 16, Issue 3, pp. 193-200, (2016) (PubMed).

Kourti, Ikonomou, Giakoumakis, Rapsomaniki, Landegren, Siniossoglou, Lygerou, Simos, Mylonis: "CK1δ restrains lipin-1 induction, lipid droplet formation and cell proliferation under hypoxia by reducing HIF-1α/ARNT complex formation." in: Cellular signalling, Vol. 27, Issue 6, pp. 1129-40, (2015) (PubMed).

Paukert, Agarwal, Cha, Doze, Kang, Bergles: "Norepinephrine controls astroglial responsiveness to local circuit activity." in: Neuron, Vol. 82, Issue 6, pp. 1263-70, (2014) (PubMed).

Pais, Milho, Almeida, Mota: "Identification of novel type III secretion chaperone-substrate complexes of Chlamydia trachomatis." in: PLoS ONE, Vol. 8, Issue 2, pp. e56292, (2013) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Background: Synonyms: Green fluorescent protein antibody.