TTC21B antibody (AA 1221-1316) (HRP)

Catalog No. ABIN1412870

Product Details anti-TTC21B Antibody (HRP)

Target: TTC21B (Tetratricopeptide Repeat Domain 21B (TTC21B))

Binding Specificity: AA 1221-1316

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TTC21B antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TTC21B

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TTC21B

Target: TTC21B (Tetratricopeptide Repeat Domain 21B (TTC21B))

Alternative Name: TTC21B (TTC21B Products)

Synonyms: 2410066K11Rik antibody, Thm1 antibody, aln antibody, mKIAA1992 antibody, RGD1565122 antibody, ATD4 antibody, IFT139 antibody, JBTS11 antibody, NPHP12 antibody, THM1 antibody, sb:cb947 antibody, tetratricopeptide repeat domain 21B antibody, Ttc21b antibody, TTC21B antibody, ttc21b antibody

Background:

Synonyms: ATD4, JBTS11, Nbla10696, NPHP12, Putative protein product of Nbla10696, Tetratricopeptide repeat protein 21B, THM1, TPR repeat protein 21B, TT21B_HUMAN, Ttc21b.

Background: The tetratricopeptide repeat (TPR) motif is a degenerate, 34 amino acid sequence found in many proteins and acts to mediate protein-protein interactions in various pathways. At the sequence level, there can be up to 16 tandem TPR repeats, each of which has a helix-turn-helix shape that stacks on other TPR repeats to achieve ligand binding specificity. TTC21B (tetratricopeptide repeat domain 21B), also known as THM1, is a 1,316 amino acid protein that contains 19 TPR repeats and belongs to the TTC21 family. Localizing to cytoplasm and cytoskeleton, TTC21B exists as two alternatively spliced isoforms and is thought to negatively regulate Shh signal transduction. TTC21B may also be involved in retrograde intraflagellar transport in cilia, and is encoded by a gene that maps to human chromosome 2q24.3.

Gene ID: 79809

UniProt: Q7Z4L5

Pathways: Hedgehog Signaling