SH3PXD2A antibody (AA 261-360) (Cy3)

Catalog No. ABIN1410387

Product Details anti-SH3PXD2A Antibody (Cy3)

Target: SH3PXD2A (SH3 and PX Domains 2A (SH3PXD2A))

Binding Specificity: AA 261-360

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3PXD2A antibody is conjugated to Cy3

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FISH

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SH3PXD2A

Target: SH3PXD2A (SH3 and PX Domains 2A (SH3PXD2A))

Alternative Name: FISH (SH3PXD2A Products)

Synonyms: FISH antibody, SH3MD1 antibody, TKS5 antibody, 2310014D11Rik antibody, AA589508 antibody, AI256723 antibody, AI413738 antibody, C230050L11 antibody, EG329070 antibody, Fish antibody, Gm5098 antibody, Sh3md1 antibody, TSK5 antibody, Tks5 antibody, sh3pxd2a antibody, si:dkey-82d4.2 antibody, SH3 and PX domains 2A antibody, SH3 and PX domains 2Aa antibody, SH3PXD2A antibody, Sh3pxd2a antibody, sh3pxd2aa antibody

Background:

Synonyms: Adapter protein TKS5, Five SH3 domain-containing protein, SH3 and PX domain-containing protein 2A, SH3 multiple domains protein 1, Sh3md1, Sh3pxd2a, SPD2A_HUMAN, TKs5, Tyrosine kinase substrate with five SH3 domains.

Background: Fish, a potential Src substrate, is a broadly expressed adaptor protein containing five SH3 domains and a phox homology (PX) domain (1). The Src family of protein tyrosine kinases act in signal transduction pathways (2-4). Src kinases vary in expression but are strongly regulated in vivo, catalytic activity is repressed by interacting with the SH3 domain (5-7). In Src-transformed fibroblasts and in normal cells treated with certain growth factors fish is tyrosine-phosphorylated (1). Treatment of cells with cytochalasin D results in rapid tyrosine phosphorylation of fish, along with activation of Src (1). Fish is likely to be involved in tyrosine kinase signaling and may have a role in cytoskeletal changes (1).