GLYT1 antibody (AA 209-285) (HRP)

Catalog No. ABIN1410152

Product Details anti-GLYT1 Antibody (HRP)

Target: GLYT1 (Glycine Transporter 1 (GLYT1))

Binding Specificity: AA 209-285

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLYT1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Glyt1/SLC6A9

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GLYT1

Target: GLYT1 (Glycine Transporter 1 (GLYT1))

Alternative Name: Glyt1/SLC6A9 (GLYT1 Products)

Synonyms: GLYT1 antibody, Glyt-1 antibody, Glyt1 antibody, GLYT-1 antibody, GLYT-1b antibody, glyt1 antibody, slc6a9 antibody, glyT-1 antibody, glyT1 antibody, solute carrier family 6 member 9 antibody, solute carrier family 6 (neurotransmitter transporter, glycine), member 9 antibody, glycine transporter 1 antibody, SLC6A9 antibody, Slc6a9 antibody, glyt1 antibody, slc6a9 antibody

Background:

Synonyms: glycine transporter 1, Glyt 1, GlyT-1 antibodyGlyT1, SC6A9_HUMAN, SLC6A9, sodium and chloride dependent glycine transporter 1, Sodium- and chloride-dependent glycine transporter 1, Solute carrier family 6 member 9.

Background: Na+/Cl- dependent neurotransmitter transporters are a superfamily of transmembrane proteins that contain 12 membrane spanning regions (1). Specifically, the highly hydrophobic Na+/Cl- dependent glycine transporters (GlyT) are crucial for the termination of neurotransmission at glycinergic synapses (2,3). Two different GlyT genes encode GlyT2 and GlyT1, which exists as two isoforms produced by alternative splicing of the same gene located on human chromosome 1p31.3 (3,4). The GlyT1 gene may be an early marker of neural development and encodes glia-specific transporter proteins (3). Although GlyT1 and GlyT2 are both expressed in the brain and spinal cord, each shows a unique pattern of expression (3,5,6). GlyT1 is found only in the white matter of the CNS, whereas GlyT2 is found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues (3,5). The anatomic distribution of GlyT2 mRNA suggests that glycine may act as a supraspinal neurotransmitter and may function as a chemical messenger outside the CNS (5).

Gene ID: 6536

UniProt: P48067