ZWILCH antibody (Biotin)

Catalog No. ABIN1404343

Product Details anti-ZWILCH Antibody (Biotin)

Target: ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZWILCH antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ZWILCH

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ZWILCH

Target: ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))

Alternative Name: ZWILCH (ZWILCH Products)

Synonyms: CG18639 antibody, CG18729 antibody, Dmel\\CG18729 antibody, Zwilch antibody, hzwilch antibody, kntc1ap antibody, KNTC1AP antibody, hZwilch antibody, 2310031L18Rik antibody, si:ch211-242m18.4 antibody, wu:fb10f09 antibody, zwilch kinetochore protein antibody, CG18729 gene product from transcript CG18729-RA antibody, protein zwilch homolog antibody, Zwilch antibody, zwilch antibody, LOC100374963 antibody, ZWILCH antibody

Background:

Synonyms: hZwilch, KNTC1AP, MGC111034, Protein zwilch homolog, ZWILC_HUMAN, zwilch,y ZWILCH protein, Zwilch, kinetochore associated, homolog.

Background: Zwilch is the human homolog of the Drosophila Zwilch protein. The Drosophila Zwilch forms a complex with both ROD Rough Deal) and ZWINT (Zeste-White 10, also designated ZW10) proteins. This complex is important for chromosome segregation because it recruits cytoplasmic Dynein to the kinetochore and plays a crucial role in the spindle checkpoint. The role of Zwilch in complex is thought to be evolutionarily conserved because the human homologs of Zwilch, ZWINT and ROD coimmunoprecipitate in a human cell line called HeLa. The human Zwilch, ZWINT and ROD complex localizes to the kinetochores at prometaphase. Mutations were discovered in Zwilch, ZWINT and ROD during a screen for mutations in alleles encoding putative chromosome instability genes in cases of human colorectal cancer. These mutations may contribute in part to the chromosomal instability phenotype of colorectal tumor cells.

Gene ID: 55055

UniProt: Q9H900