PDZRN3 antibody (AbBy Fluor® 555)

Catalog No. ABIN1401365

Product Details anti-PDZRN3 Antibody (AbBy Fluor® 555)

Target: PDZRN3 (PDZ Domain Containing Ring Finger 3 (PDZRN3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDZRN3 antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SEMCAP3/LNX3

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDZRN3

Target: PDZRN3 (PDZ Domain Containing Ring Finger 3 (PDZRN3))

Alternative Name: SEMCAP3 (PDZRN3 Products)

Synonyms: MGC80196 antibody, pdzrn3 antibody, LNX3 antibody, SEMACAP3 antibody, 1110020C07Rik antibody, AI429718 antibody, AL023082 antibody, Semcap3 antibody, PDZ domain containing ring finger 3 antibody, PDZ domain containing ring finger 3 L homeolog antibody, PDZ domain containing RING finger 3b antibody, E3 ubiquitin-protein ligase PDZRN3 antibody, PDZ domain containing RING finger 3 antibody, PDZRN3 antibody, pdzrn3.L antibody, pdzrn3b antibody, LOC100228989 antibody, pdzrn3 antibody, Pdzrn3 antibody

Background:

Synonyms: Ligand of Numb protein X 3, likely ortholog of mouse semaF cytoplasmic domain associated protein 3, LNX3, PDZ domain containing ring finger 3, Protein SEMACAP3, SEMACAP3, Semaphorin cytoplasmic domain associated protein, PZRN3_HUMAN.

Background: PDZRN3 contains a RING-finger motif in its N-terminal region, two PDZ domains in its central region and a consensus-binding motif for PDZ domains at its C-terminus. It was identified in silico as a homolog of the protein known as LNX1 or SEMCAP1, which possesses ubiquitin ligase activity and binds the membrane protein Semaphorin 4C. However, PDZRN3 itself has not previously been characterized. We have now evaluated the properties and functions of PDZRN3. The PDZRN3 gene was shown to be expressed in various human tissues including the heart, skeletal muscle and liver and its expression in mouse skeletal muscle was developmentally regulated. Both the differentiation of C2C12 mouse skeletal myoblasts into myotubes and injury-induced muscle regeneration in vivo were found to be accompanied by up-regulation of PDZRN3. The differentiation-associated increase in the expression of PDZRN3 in C2C12 cells follows that of myogenin and precedes that of myosin heavy chain. Depletion of PDZRN3 by RNA interference inhibited the formation of myotubes as well as the associated up-regulation of myosin heavy chain in C2C12 cells. Our data suggest that PDZRN3 plays an essential role in the differentiation of myoblasts into myotubes by acting either downstream or independently of myogenin.

Gene ID: 23024

Pathways: Skeletal Muscle Fiber Development