FBXO25 antibody (AbBy Fluor® 350)

Catalog No. ABIN1399347

Product Details anti-FBXO25 Antibody (AbBy Fluor® 350)

Target: FBXO25 (F-Box Protein 25 (FBXO25))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO25 antibody is conjugated to AbBy Fluor® 350

Application: Western Blotting (WB)

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FBXO25

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FBXO25

Target: FBXO25 (F-Box Protein 25 (FBXO25))

Alternative Name: FBXO25 (FBXO25 Products)

Synonyms: FBXO25 antibody, fbxo32 antibody, MGC108443 antibody, DKFZp469H2437 antibody, zgc:100907 antibody, FBX25 antibody, 9130015I06Rik antibody, AI649137 antibody, Fbx25 antibody, F-box protein 25 antibody, F-box protein 25 L homeolog antibody, FBXO25 antibody, fbxo25 antibody, fbxo25.L antibody, Fbxo25 antibody

Background:

Synonyms: F box only protein 25, F box protein 25, F box protein Fbx25, F-box only protein 25, FBX25, MGC20256, MGC51975, OTTHUMP00000115399,

Background: FBXO25 is a member of the F box protein family which is characterized by an approximately 40 amino acid motif, the F box. The F box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1 cullin F box), which function in phosphorylation dependent ubiquitination. The F box proteins are divided into 3 classes: Fbws containing WD 40 domains, Fbls containing leucine rich repeats, and Fbxs containing either different protein protein interaction modules or no recognizable motifs. FBXO25 belongs to the Fbxs class. There are three named isoforms produced by alternative splicing.FBXO25, also known as FBX25, is a 367 amino acid protein that contains one C-terminal F-box domain and belongs to the Fbx class of the F-box family of proteins. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune response, signaling cascades and developmental processes) through the targeting of proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkB-a and b-catenin, for degradation by the proteasome after ubiquitination. Expressed at high levels in brain, FBXO25 localizes predominantly to the nucleus and directly interacts with Skp1 p19 and CUL-1. Disruption of the gene encoding FBXO25 can lead to X-linked mental retardation.

Gene ID: 26260