Rubisco antibody (Biotin)
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- Target See all Rubisco (RBCS) products
- Rubisco (RBCS)
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Reactivity
- Arabidopsis thaliana
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Rubisco antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human, Plant
- Cross-Reactivity (Details)
- Arabidopsis thaliana,
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from Arabidopsis thaliana RUBISCO
- Isotype
- IgG
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- Application Notes
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WB 1:100-1000
IHC-P 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C for 12 months.
- Expiry Date
- 12 months
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- Target
- Rubisco (RBCS)
- Alternative Name
- RUBISCO (RBCS Products)
- Background
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Synonyms: rbcL, RBL_SPIOL, Ribulose bisphosphate carboxylase large chain, RuBisCO large subunit, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit.
Background: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.Sequence similarities: Belongs to the RuBisCO large chain family. Type I subfamily.Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.
- Gene ID
- 844754
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