HIV-1 Gag antibody (AA 351-450) (AbBy Fluor® 488)

Catalog No. ABIN1397217

Product Details anti-HIV-1 Gag Antibody (AbBy Fluor® 488)

Target: HIV-1 Gag (GAG_HIV) (HIV-1 Gag Polyprotein (GAG_HIV))

Binding Specificity: AA 351-450

Reactivity: Human Immunodeficiency Virus (HIV), Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIV-1 Gag antibody is conjugated to AbBy Fluor® 488

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Specificity: This antibody will recognize the full Gag protein and Nucleocapsid protein p7 after cleavage

Cross-Reactivity: Virus

Cross-Reactivity (Details): HIV

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from HIV1 Nucleocapsid protein p7

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HIV-1 Gag

Target: HIV-1 Gag (GAG_HIV) (HIV-1 Gag Polyprotein (GAG_HIV))

Alternative Name: HIV1 Gag Protein (GAG_HIV Products)

Target Type: Viral Protein

Background:

Synonyms: HIV1 Pr55Gag, Human immunodeficiency virus 1, Gag protein, Nucleocapsid protein p7,

Background: HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1.