LIG4 antibody (AA 51-150) (Biotin)

Catalog No. ABIN1396062

Product Details anti-LIG4 Antibody (Biotin)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Binding Specificity: AA 51-150

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG4 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA Ligase IV

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: DNA Ligase IV/LIG4 (LIG4 Products)

Synonyms: zgc:165595 antibody, lig4 antibody, DNA LIGASE IV antibody, LIG4 antibody, lig4-A antibody, 5830471N16Rik antibody, tiny antibody, DNL4 antibody, ligase IV, DNA, ATP-dependent antibody, DNA ligase 4 antibody, ATP-dependent DNA ligase implicated in dsDNA break repair via nonhomologous end-joining antibody, DNA ligase IV antibody, ligase IV, DNA, ATP-dependent L homeolog antibody, lig4 antibody, LIG4 antibody, LOC100212302 antibody, CpipJ_CPIJ005161 antibody, PTRG_09982 antibody, MCYG_05151 antibody, LOC657210 antibody, ATLIG4 antibody, lig4.L antibody, Lig4 antibody

Background:

Synonyms: LIG4S, DNA ligase 4, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4

Background: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Gene ID: 3981

UniProt: P49917

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response