EN2 antibody (AA 281-333) (Biotin)

Catalog No. ABIN1391947

Product Details anti-EN2 Antibody (Biotin)

Target: EN2 (Engrailed Homeobox 2 (EN2))

Binding Specificity: AA 281-333

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EN2/Engrailed 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

References for anti-EN2 antibody (Biotin) (ABIN1391947)

De Paoli, Gogalic, Sauer, Preininger, Pandha, Simpson, Horvath, Marquette: "Multiplatform Biomarker Discovery for Bladder Cancer Recurrence Diagnosis." in: Disease markers, Vol. 2016, pp. 4591910, (2016) (PubMed).

Target Details for EN2

Target: EN2 (Engrailed Homeobox 2 (EN2))

Alternative Name: EN2/Engrailed 2 (EN2 Products)

Synonyms: EN2 antibody, en antibody, en2 antibody, BB131122 antibody, En-2 antibody, RGD1561842 antibody, Gg-En-2 antibody, engrailed homeobox 2 antibody, engrailed homeobox antibody, engrailed 2 antibody, EN2 antibody, en antibody, En2 antibody

Background:

Synonyms: Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2

Background: The engrailed-2 gene, EN2, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homologs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expression of a-synuclein, a gene that is genetically linked to Parkinson?s disease. During early brain development mouse EN-2 is expressed in a broad band across most of the mid-hindbrain region. EN-2 is also expressed in mouse myoblasts and has been assiciated with cerebellar hypoplasia.

Gene ID: 2020

UniProt: P19622