UNC5B antibody (AA 188-260) (Biotin)

Catalog No. ABIN1391587

Product Details anti-UNC5B Antibody (Biotin)

Target: UNC5B (Netrin receptor UNC5B (UNC5B))

Binding Specificity: AA 188-260

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNC5B antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UNC5B

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for UNC5B

Target: UNC5B (Netrin receptor UNC5B (UNC5B))

Alternative Name: UNC5B/UNC5H2 (UNC5B Products)

Synonyms: unc5h2 antibody, 6330415E02Rik antibody, A630020F16 antibody, D10Bwg0792e antibody, Unc5h2 antibody, UNC5H2 antibody, p53RDL1 antibody, UNC5C antibody, UNC5B antibody, p53rdl1 antibody, unc-5 netrin receptor B antibody, unc-5 netrin receptor B L homeolog antibody, unc5b antibody, Unc5b antibody, UNC5B antibody, unc5b.L antibody

Background:

Synonyms: Netrin receptor UNC5B, Netrin receptor UNC5B Precursor, p53 regulated receptor for death and le, p53 regulated receptor for death and le protein 1, p53-regulated receptor for death and le protein 1, p53RDL1, Protein unc-5 homolog 2, Protein unc-5 homolog B, Protein XUNC 5, Transmembrane receptor Unc5H2, Unc 5 homolog 2, Unc 5 homolog B, UNC 5B, Unc5 C.elegans homolog b, Unc5b, UNC5B_HUMAN, UNC5H2.

Background: The UNC5H family of proteins act as transmembrane receptors for netrin-1 and play a crucial role in axon guidance and migration of neural cells. In fact, UNC5H receptors express widely in cells that migrate, where they bind the G protein G Alpha 1-2 to inhibit G protein signaling. Additionally, UNC5H receptors induce apoptosis when cleaved by a caspase, producing an intracellular fragment containing a death domain, but this activity is blocked by the binding of netrin-1. The expression of UNC5H receptors is down-regulated in multiple cancers, including colorectal, breast, ovary, uterus, stomach, lung, and kidney cancers. Hence, in the absence of netrin-1, UNC5H receptors act as tumor suppressors by inhibiting anchorage-independent growth and invasion, but mutation of these receptors provides a potential mechanism for tumorigenicity. UNC5H2, also designated unc-5 homolog B or p53-regulated receptor for death and life protein 1 (p53RDL1) is highly expressed in brain with lower levels of expression observed in developing lung, cartilage, kidney and hematopoietic and immune tissues.