GRAF antibody (AA 1-100) (Biotin)

Catalog No. ABIN1390153

Product Details anti-GRAF Antibody (Biotin)

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Binding Specificity: AA 1-100

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRAF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GRAF1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for GRAF

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Alternative Name: GRAF1 (ARHGAP26 Products)

Synonyms: ARHGAP26 antibody, GRAF antibody, GRAF1 antibody, OPHN1L antibody, OPHN1L1 antibody, 1810044B20Rik antibody, 2610010G17Rik antibody, 4933432P15Rik antibody, AI853435 antibody, mKIAA0621 antibody, Rho GTPase activating protein 26 antibody, ARHGAP26 antibody, Arhgap26 antibody

Background:

Synonyms: arhgap 26, ARHGAP26, FLJ42530, GRAF, GRAF1, GTPase regulator associated with focal adhesion kinase, GTPase regulator associated with focal adhesion kinase pp125FAK, KIAA0621, oligophrenin 1 like protein, Oligophrenin-1-like protein, oligophrenin1like protein, OPHN1L, OPHN1L1, RHG26_HUMAN, Rho GTPase activating protein 26, Rho GTPase activating protein26, Rho GTPase-activating protein 26, Rho-type GTPase-activating protein 26.

Background: Cellular signaling by G-proteins is down-regulated by GTPase-activating proteins (GAPs), which increase the rate of GTP hydroylsis. The GTPase regulator associated with focal adhesion kinase (GRAF) has GAP activity toward Rho A and Cdc42, but not Rac1. GRAF is ubiquitously expressed with high levels in heart and brain. Expression of GRAF causes clearing of stress fibers and formation of long actin based filopodial-like extensions. Fusion of MLL with GRAF, MLL/GRAF, is included in a rare genetic subgroup of acute myeloid leukemia (AML) cases.