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AAV VP1 antibody (AbBy Fluor® 488)

AAV VP1 Reactivity: Adeno-Associated Virus (AAV) IF (cc), IF (p) Host: Rabbit Polyclonal AbBy Fluor® 488
Catalog No. ABIN1388674
  • Target See all AAV VP1 products
    AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))
    Reactivity
    Adeno-Associated Virus (AAV)
    Host
    • 13
    Rabbit
    Clonality
    • 13
    Polyclonal
    Conjugate
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AAV VP1 antibody is conjugated to AbBy Fluor® 488
    Application
    • 11
    • 11
    • 3
    • 3
    • 3
    • 1
    Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Specificity
    This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13
    Cross-Reactivity
    Virus
    Cross-Reactivity (Details)
    AAV5
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1
    Isotype
    IgG
  • Application Notes
    IF(IHC-P) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))
    Alternative Name
    Adeno-Associated Virus Capsid Protein VP1 (AAV VP1 Products)
    Target Type
    Viral Protein
    Background

    Synonyms: capsid protein Adeno-associated 5 virus, capsid protein [Adeno-associated virus - 5], capsid protein AAV5, Parvovirus coat protein VP1, capsid protein.

    Background: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus

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