LEO1 antibody (AA 451-550) (AbBy Fluor® 647)
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- Target See all LEO1 Antibodies
- LEO1 (Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1))
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Binding Specificity
- AA 451-550
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This LEO1 antibody is conjugated to AbBy Fluor® 647
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Cross-Reactivity
- Rat
- Predicted Reactivity
- Human,Mouse,Dog,Cow,Pig,Horse,Chicken,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human LEO1
- Isotype
- IgG
- Top Product
- Discover our top product LEO1 Primary Antibody
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- Application Notes
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- LEO1 (Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1))
- Alternative Name
- LEO1 (LEO1 Products)
- Synonyms
- LEO1 antibody, RDL antibody, Gm185 antibody, zgc:77374 antibody, LEO1 homolog, Paf1/RNA polymerase II complex component antibody, Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) antibody, RNA polymerase-associated protein LEO1 antibody, LEO1 homolog, Paf1/RNA polymerase II complex component S homeolog antibody, Leo1, Paf1/RNA polymerase II complex component antibody, LEO1 antibody, leo1 antibody, LOC100466503 antibody, leo1.S antibody, Leo1 antibody, LOC415420 antibody, LOC478310 antibody
- Background
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Synonyms: RDL, RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, LEO1
Background: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling.
- Gene ID
- 123169
- UniProt
- Q8WVC0
- Pathways
- Stem Cell Maintenance
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