UBE2E2 antibody (AA 1-100)

Catalog No. ABIN1388005

Product Details anti-UBE2E2 Antibody

Target: UBE2E2 (Ubiquitin-Conjugating Enzyme E2E 2 (UBE2E2))

Binding Specificity: AA 1-100

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2E2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2E2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for UBE2E2

Target: UBE2E2 (Ubiquitin-Conjugating Enzyme E2E 2 (UBE2E2))

Alternative Name: UBE2E2 (UBE2E2 Products)

Synonyms: ubch8 antibody, UBCH8 antibody, wu:fb38h07 antibody, zgc:92467 antibody, BC016265 antibody, ubiquitin-conjugating enzyme E2E 2 antibody, ubiquitin conjugating enzyme E2E 2 L homeolog antibody, ubiquitin conjugating enzyme E2E 2 antibody, Ube2e2 protein antibody, ubiquitin conjugating enzyme E2 E2 antibody, UBE2E2 antibody, ube2e2.L antibody, ube2e2 antibody, Ube2e2 antibody

Background:

Synonyms: UB2E2_HUMAN, UBC4/5 homolog yeast, UBCH 8, UbcH8, Ube2e2, Ubiquitin carrier protein E2, Ubiquitin conjugating enzyme E2 E2, Ubiquitin conjugating enzyme E2E 2 homologous to yeast UBC4/5, Ubiquitin conjugating enzyme E2E 2 UBC4/5 homolog yeast, Ubiquitin conjugating enzyme E2E 2, Ubiquitin protein ligase E2, Ubiquitin-conjugating enzyme E2 E2, Ubiquitin-protein ligase E2.

Background: Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). The first step in the ubiquitination process requires the ATP-dependent activation of the ubiquitin C-terminus and the assembly of multi-ubiquitin chains by the E1 enzyme. The ubiquitin chain is then conjugated to the E2 enzyme to generate an intermediate ubiquitin-E2 complex. The E3 enzyme then catalyzes the transfer of ubiquitin from E2 to the appropriate protein substrate, thereby targeting that substrate for degradation. A wide range of enzymes facilitate this proteolytic ubiquitin pathway, one of which is UBE2E2 (also known as UBCH8 in human), which functions as an E2 enzyme and catalyzes the ATP-dependent covalent attachment of ubiquitin to target proteins, thereby playing an important role in protein degradation.

Gene ID: 7325