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CD8 alpha antibody (AA 51-150)

Name: CD8 alpha antibody (AA 51-150)
SKU: ABIN1387948
Price: 384.62 USD
Availability: InStock

CD8A Reactivity: Human, Rat, Chicken FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded rat spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (CD8 alpha antibody (AA 51-150))

IHC (p)

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (ABIN1387948) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (CD8 alpha antibody (AA 51-150))

IF (p)

3 images

(2 references)

Catalog No. ABIN1387948

Datasheet as PDF

Target See all CD8 alpha (CD8A) Antibodies

CD8 alpha (CD8A) (CD8a Molecule (CD8A))
Binding Specificity
15

15

10

9

9

9

8

7

6

6

5

5

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 51-150
Reactivity
184

105

46

38

15

7

3

2

2

1

1

1

1

1

1

1

1
Human, Rat, Chicken
Host
147

126

92
Rabbit
Compatible Secondaries
Clonality
259

102

1
Polyclonal
Conjugate
115

43

35

27

26

10

8

7

7

6

5

5

5

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 alpha antibody is un-conjugated
Application
287

124

85

64

58

42

19

17

14

13

13

9

7

6

6

6

5

3

3

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity

Chicken, Human, Rat

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from rat CD8 alpha

Isotype

IgG

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CD8A Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 8H8A8 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Mouse FACS, IP, IHC (p), IHC (fro), ICC Host: Rat Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Mouse FACS, IP, IHC (p), IHC (fro), ICC, Func Host: Rat Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody (FITC)
CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 FITC

anti-CD8a Molecule (CD8A) antibody (PE)
CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 PE

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Human IHC, WB, IP, ELISA Host: Rabbit Monoclonal 2F2 unconjugated Recombinant Antibody

anti-CD8a Molecule (CD8A) (AA 22-182) antibody
CD8A Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 6A9A5 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Human IHC, StM, ISt Host: Mouse Monoclonal C8-468 unconjugated

anti-CD8a Molecule (CD8A) (AA 28-196) antibody
CD8A Reactivity: Mouse, Rat FACS, IHC, WB, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-CD8a Molecule (CD8A) (AA 150-180) antibody
CD8A Reactivity: Human FACS, IHC, WB, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Savvatis, Müller, Fröhlich, Pappritz, Zietsch, Hamdani, Grote, Schieffer, Klingel, Van Linthout, Linke, Schultheiss, Tschöpe: "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." in: Basic research in cardiology, Vol. 109, Issue 6, pp. 449, (2014) (PubMed).

Cheng, Guo, Qiao, Liu, Nie, Li, Wang, Jiang: "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." in: Diagnostic pathology, Vol. 8, pp. 168, (2013) (PubMed).
Target

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name

CD8 alpha (CD8A Products)

Synonyms

cd8 antibody, LOC100136450 antibody, BB154331 antibody, Ly-2 antibody, Ly-35 antibody, Ly-B antibody, Lyt-2 antibody, zgc:136643 antibody, CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, RHACD8-4 antibody, CD8a molecule antibody, uncharacterized LOC100125537 antibody, CD8 alpha antibody, CD8 antigen, alpha chain antibody, T-cell surface glycoprotein CD8 alpha chain antibody, Cd8a antibody, LOC100125537 antibody, LOC100136450 antibody, CD8A antibody, cd8a antibody, LOC100126556 antibody

Background

Synonyms: T-cell surface glycoprotein CD8 alpha chain, CD8 antigen 32 kDa chain, OX-8 membrane antigen, CD8a
Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Gene ID

24930

UniProt

P07725

Pathways

TCR Signaling