HOXD9 antibody (AA 251-352)

Catalog No. ABIN1387750

Product Details anti-HOXD9 Antibody

Target: HOXD9 (Homeobox D9 (HOXD9))

Binding Specificity: AA 251-352

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOXD9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HOXD9

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HOXD9

Target: HOXD9 (Homeobox D9 (HOXD9))

Alternative Name: HOXD9 (HOXD9 Products)

Synonyms: Hox-4.4 antibody, Hox-5.2 antibody, Xhoxd9 antibody, HOX4 antibody, HOX4C antibody, Hox-4.3 antibody, hoxd9 antibody, z-28 antibody, hoxd9a antibody, homeobox D9 antibody, homeobox D9 S homeolog antibody, homeo box D9 antibody, homeobox D9a antibody, Hoxd9 antibody, HOXD9 antibody, hoxd9.S antibody, hoxd9a antibody, hoxd9 antibody

Background:

Synonyms: homeobox D9, Homeobox protein Hox D9, Hox 4.3, Hox 5.2, HOX4, HOX4C, RP23 313J15.10, HXD9_HUMAN.

Background: The Hox proteins play a role in patterns of embryonic development and cellular differentiation by regulating downstream target genes. In vivo, the HoxD9 protein interacts with the autoregulatory and cross-regulatory enhancers of the murine HoxB1 and human HoxD9 genes. Specifically, the HoxD9 protein interacts with the human control region (HCR) of the HoxD9 gene, thus inducing transcription of the HoxD9 promoter. HoxD9 may be a multifunctional transcriptional regulator, as it contains different activation domains. Activation of HoxD9 depends on the structure of the target regulatory element, and results in differential cofactor interaction. The HoxD9 protein is expressed in the early stages of mouse joint development, primarily in the articular cartilage. HoxD9 transcripts are also detected in the synovial tissue of arthritic mice, but not in that of normal mice, suggesting that HoxD9 may have a role in the pathology of arthritis. Furthermore, the HoxD9 protein is highly expressed in the synoviocytes of patients with rheumatoid arthritis (RA), but not in osteoarthritis patients. The human HoxD9 protein is also differentially expressed in the human cervical cancer cell line HeLa, but is not expressed in the normal cervix and may thus play a role in tumorigenesis.