ADAM12 antibody (AA 251-350)

Catalog No. ABIN1387748

Product Details anti-ADAM12 Antibody

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Binding Specificity: AA 251-350

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ADAM12/MLTN

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ADAM12 antibody (ABIN1387748)

Branco, da Costa, Abe, Kataoka, Pinheiro, Alves Júnior: "HIF-1α, NOTCH1, ADAM12, and HB-EGF are overexpressed in mucoepidermoid carcinoma." in: Oral surgery, oral medicine, oral pathology and oral radiology, Vol. 127, Issue 1, pp. e8-e17, (2019) (PubMed).

Target Details for ADAM12

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Alternative Name: ADAM12 (ADAM12 Products)

Background:

Synonyms: MLTN, A disintegrin and metalloproteinase domain 12, A disintegrin and metalloproteinase domain 12, ADA12_HUMAN, ADAM 12, ADAM metallopeptidase domain 12, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, MCMPMltna, Meltrin alpha, Meltrin-alpha, MLTN, MLTNA, OTTHUMP00000046766.

Background: ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNF?(tumor necrosis factor ?, and modulation of the neurogenic function of Notch and Delta (1-3). ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail (1-3). ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone, and muscle, and catalyze proteolysis, adhesion, fusion, and intracellular signaling (3). ADAM 12 (Meltrin-a) is produced as 2 differentially spliced isoforms, a 718 amino acid secreted form (ADAM12S) and a 881 amino acid membrane-bound form (ADAM12L), and is involved in egg-sperm fusion (4-6).

Pathways: EGFR Signaling Pathway