PHF21A antibody (AA 351-450)

Catalog No. ABIN1387678

Product Details anti-PHF21A Antibody

Target: PHF21A (PHD Finger Protein 21A (PHF21A))

Binding Specificity: AA 351-450

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHF21A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PHF21A

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PHF21A

Target: PHF21A (PHD Finger Protein 21A (PHF21A))

Alternative Name: BHC80/PHF21A (PHF21A Products)

Background:

Synonyms: BHC80a, BM-006, BRAF35-HDAC complex protein BHC80, BRAF35-HDAC complex protein BHC80, BRAF35-HDAC complex protein BHC80, BRAF35/HDAC2 complex 80 kDa, KIAA1696, PF21A_HUMAN, PHD finger protein 21A, PHF21A.

Background: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. PHF21A is a 680 amino acid nuclear protein that contains one PHD-type zinc finger and one A.T hook DNA-binding domain, suggesting involvement in transcriptional regulation events. PHF21A is a component of the BHC complex, which is responsible for repressing transcription of neuron-specific genes in non-neuronal cells. The BHC complex acts as a chromatin modifier that deacetylates and demethylates specific sites on histones. PHF21A may act as a scaffold within the BHC complex. Predominantly expressed in brain, three isoforms of PHF21A exist as a result of alternative splicing events.

Gene ID: 51317

Pathways: Feeding Behaviour