DEPTOR antibody (AA 56-170)

Catalog No. ABIN1387597

Product Details anti-DEPTOR Antibody

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Binding Specificity: AA 56-170

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEPTOR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DEPTOR/DEPDC6

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-DEPTOR antibody (ABIN1387597)

Liu, Cai, Zhang, Liu, Luo, Xing, Du: "Deacetylation of NAT10 by Sirt1 promotes the transition from rRNA biogenesis to autophagy upon energy stress." in: Nucleic acids research, Vol. 46, Issue 18, pp. 9601-9616, (2019) (PubMed).

Liu, Zhang, Liu, Li, Zhang, Li, Liu, Huang, Shen, Gu, Gao, Wu, Wu: "High DEPTOR expression correlates with poor prognosis in patients with esophageal squamous cell carcinoma." in: OncoTargets and therapy, Vol. 8, pp. 3449-55, (2015) (PubMed).

Target Details for DEPTOR

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Alternative Name: Deptor (DEPTOR Products)

Synonyms: DEP.6 antibody, DEPDC6 antibody, 4731402B04Rik antibody, 9130412E02Rik antibody, D15Ertd336e antibody, D15Ertd597e antibody, Depdc6 antibody, Depdc6-001 antibody, Depdc6-002 antibody, R75183 antibody, mKIAA4200 antibody, RGD1561030 antibody, RAPGEF4 antibody, DEP domain containing MTOR interacting protein antibody, DEP domain containing MTOR-interacting protein antibody, DEPTOR antibody, Deptor antibody

Background:

Synonyms: DEP.6, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6, DEPTOR

Background: DEPTOR (DEP domain containing MTOR-interacting protein), also known as DEP.6 or DEPDC6 (DEP domain-containing protein 6), is a 409 amino acid protein that negatively regulates mTORC1 and mTORC2 pathways. DEPTOR interacts with FRAP via its PDZ domain, and undergoes post-translational phosphorylation. Containing two DEP domains and one PDZ (DHR) domain, DEPTOR is encoded by a gene that maps to human chromosome 8q24.12. Chromosome 8 consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

Gene ID: 64798

UniProt: Q8TB45

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Cell Size, BCR Signaling, Warburg Effect