FOXN1 antibody (AA 321-420)

Catalog No. ABIN1387592

Product Details anti-FOXN1 Antibody

Target: FOXN1 (Forkhead Box N1 (FOXN1))

Binding Specificity: AA 321-420

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FOXN1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FOXN1 antibody (ABIN1387592)

Volpi, Yamazaki, Brauer, van Rooijen, Hayashida, Slavotinek, Sun Kuehn, Di Rocco, Rivolta, Bortolomai, Du, Felgentreff, Ott de Bruin, Hayashida, Freedman, Marcovecchio, Capuder, Rath, Luche, Hagedorn et al.: "EXTL3 mutations cause skeletal dysplasia, immune deficiency, and developmental delay. ..." in: The Journal of experimental medicine, Vol. 214, Issue 3, pp. 623-637, (2017) (PubMed).

Target Details for FOXN1

Target: FOXN1 (Forkhead Box N1 (FOXN1))

Alternative Name: FOXN1 (FOXN1 Products)

Background:

Synonyms: WHN, RONU, FKHL20, Forkhead box protein N1, Winged-helix transcription factor nude, FOXN1

Background: Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008].

Gene ID: 8456

UniProt: O15353