Proteoglycan 4 antibody (AA 1151-1250)

Catalog No. ABIN1387463

Product Details anti-Proteoglycan 4 Antibody

Target: Proteoglycan 4 (PRG4)

Binding Specificity: AA 1151-1250

Reactivity: Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Proteoglycan 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Mouse, Rabbit, Rat

Predicted Reactivity: Human,Dog,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Lubricin/SZP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Proteoglycan 4

Target: Proteoglycan 4 (PRG4)

Alternative Name: Lubricin/SZP (PRG4 Products)

Synonyms: CACP antibody, HAPO antibody, JCAP antibody, MSF antibody, SZP antibody, bG174L6.2 antibody, lubricin antibody, prg4 antibody, si:dkey-97o5.3 antibody, zgc:122988 antibody, PRG4 antibody, DOL54 antibody, proteoglycan 4 antibody, proteoglycan 4b antibody, proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) antibody, PRG4 antibody, Prg4 antibody, prg4b antibody

Background:

Synonyms: Superficial zone proteoglycan, articular superficial zone protein, bG174L6.2, CACP, camptodactyly arthropathy coxa vara pericarditis syndrome gene, FLJ32635, HAPO, Jacobs camptodactyly-arthropathy-pericarditis syndrome gene, JCAP, megakaryocyte stimulating factor, MSF, PRG 4, PRG4, proteoglycan 4, Proteoglycan4, Superficial zone proteoglycan, SZP.

Background: Lubricin, also designated proteoglycan-4 or megakaryocyte stimulating factor, is important for boundary lubrication within articulating joints. It is a disulfide-linked homodimer (between Cysteine 1146 and Cysteine 1403) that is essential for protein cleavage. Lubricin inhibits synovial cell adhesion to the cartilage surface, but also prevents the deposition of proteins from synovial fluid onto cartilage. Lubricin is highly expressed in cartilage, liver and synovial tissue. Defects in the gene encoding for lubricin can cause Jakobs syndrome, also designated camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP). CACP is an autosomal recessive disorder characterized by joint failure associated with noninflammatory synoviocyte hyperplasia and subinitimal fibrosis of the synovial capsule. Lubricin undergoes different levels of glycosylation and may be detected at varying molecular weights.

Gene ID: 10216

UniProt: Q92954