PHOX2A antibody (AA 41-140)

Catalog No. ABIN1387382

Product Details anti-PHOX2A Antibody

Target: PHOX2A (Paired-Like Homeobox 2a (PHOX2A))

Binding Specificity: AA 41-140

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHOX2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PHOX2A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PHOX2A

Target: PHOX2A (Paired-Like Homeobox 2a (PHOX2A))

Alternative Name: PHOX2A/CFEOM2 (PHOX2A Products)

Synonyms: ARIX antibody, CFEOM2 antibody, FEOM2 antibody, NCAM2 antibody, PMX2A antibody, Arix antibody, Phox2 antibody, Pmx2 antibody, Pmx2a antibody, arix antibody, sou antibody, zgc:77364 antibody, phox2 antibody, xphox2a antibody, paired like homeobox 2a antibody, paired-like homeobox 2a antibody, paired like homeobox 2a S homeolog antibody, PHOX2A antibody, Phox2a antibody, phox2a antibody, phox2a.S antibody

Background:

Synonyms: Aristaless homeobox Drosophila fibrosis of extraocular muscles congenital 2 autosomal recessive, Aristaless homeobox gene homolog Drosophila, Aristaless homeobox homolog, Aristaless homeobox protein homolog, ARIX 1 homeodomain protein, ARIX, Arix homeodomain protein, ARIX1 homeodomain protein, CFEOM 2, CFEOM2, FEOM 2, FEOM2, Fibrosis of extraocular muscles congenital 2 autosomal recessive, MGC52227, NCAM 2, NCAM2, Paired like aristaless Homeobox 2A, Paired like homeobox 2a, Paired mesoderm homeobox 2a, Paired mesoderm homeobox protein 2A, Paired-like homeobox 2A, PHOX 2A, Phox2, Phox2a, PHX2A_HUMAN, Pmx 2a, Pmx2, Pmx2a.

Background: Phox2a (also designated Arix1) and Phox2b are closely related, paired-homeodomain transcription factors that are necessary for neuronal differentiation throughout the developing sympathetic, parasympathetic and enteric ganglia. All enteric nervous system cells evolve from the neural crest, and all cells that are undifferentiated initially express Phox2b. The cells that begin to differentiate along a neuronal lineage continue to express Phox2b, and begin to express Phox2a. Phox2b is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain. In contrast, Phox2a controls only the differentiation of the main noradrenergic center of the brain, the locus ceruleus. Both Phox2a and Phox2b are crucial for the regulation of endogenous tyrosine hydroxylase and dopamine-beta hydroxylase, which are transiently expressed in neural crest cells. In addition, Phox2 proteins are sufficient to promote sympathetic neuron generation. The gene which encodes Phox2a maps to human chromosome 11q13.3-q13.4.