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CD19 antibody (AA 151-250)

Name: CD19 antibody (AA 151-250)
SKU: ABIN1387313
Price: 384.62 USD
Availability: InStock

CD19 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD19 Polyclonal Antibody, PE-Cy5 Conjugated . (CD19 antibody (AA 151-250))

FACS

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm. (CD19 antibody (AA 151-250))

ELISA

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD19 Polyclonal Antibody, Unconjugated (ABIN1387313) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD19 antibody (AA 151-250))

IHC

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD19 Polyclonal Antibody, Unconjugatedused at 1:200 dilution for 40 minutes at 37°C. (CD19 antibody (AA 151-250))

IF (p)

Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (CD19 antibody (AA 151-250))

Paraformaldehyde-fixed, paraffin embedded fetal mouse brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD19 antibody (AA 151-250))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD19 antibody (AA 151-250))

IHC (p)

7 images

Catalog No. ABIN1387313

Datasheet as PDF

Target See all CD19 Antibodies

CD19 (CD19 Molecule (CD19))
Binding Specificity
21

16

15

14

10

7

4

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 151-250
Reactivity
272

150

38

28

19

10

6

5

3

3

2

2

2

2

2

2

2

2
Human, Mouse, Rat
Host
169

123

46

4
Rabbit
Compatible Secondaries
Clonality
225

112

3
Polyclonal
Conjugate
115

22

22

20

17

8

8

8

8

6

5

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD19 antibody is un-conjugated
Application
254

108

66

63

53

49

21

21

14

14

10

9

5

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Pig,Guinea Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD19

Isotype

IgG
anti-CD19 Molecule (CD19) (AA 20-291) antibody
CD19 Reactivity: Human FACS, WB, IHC, IF, ELISA Host: Mouse Monoclonal 8G11B7 unconjugated

anti-CD19 Molecule (CD19) (C-Term) antibody
CD19 Reactivity: Human, Mouse, Monkey, Rat, Sheep, Pig, Cow, Guinea Pig, Dog, Avian, Cat, Chicken, Donkey, Goat, Hamster, Horse, Rabbit WB Host: Goat Polyclonal unconjugated

anti-CD19 Molecule (CD19) (pTyr531) antibody
CD19 Reactivity: Human, Mouse FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 485-556) antibody
CD19 Reactivity: Human, Mouse, Rat, Sheep FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 96-281) antibody
CD19 Reactivity: Human FACS, IHC, ELISA, Coat, StM Host: Mouse Monoclonal CD19-3116 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, WB, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 143-172) antibody
CD19 Reactivity: Human FACS, WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, IP Host: Mouse Monoclonal LT19 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, ICC Host: Mouse Monoclonal 4G7 unconjugated

anti-CD19 Molecule (CD19) (AA 160-258) antibody
CD19 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

CD19 (CD19 Molecule (CD19))

Alternative Name

CD19 (CD19 Products)

Background

Synonyms: B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19
Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Gene ID

930

UniProt

P15391

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway