Dihydrofolate Reductase antibody

Catalog No. ABIN1387277

Product Details anti-Dihydrofolate Reductase Antibody

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Purification: purified by Protein A and peptide affinity chromatography

Immunogen: KLH conjugated synthetic peptide derived from human DHFR

Isotype: IgG

Application Details

Application Notes: WB (1:100-500),IHC-P (1:100-500),FACS (1:50-200),IF (p) (1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 µg/µL

Buffer: Aqueous buffered solution containing 100 µg/mL BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for 12 months

Expiry Date: 12 months

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR (DHFR Products)

Background: DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

Molecular Weight: 21kDa

Gene ID: 1719

HGNC: 0

Pathways: Mitotic G1-G1/S Phases