DCLK1 antibody (AA 151-250)

Catalog No. ABIN1387266

Product Details anti-DCLK1 Antibody

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Binding Specificity: AA 151-250

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DCAMKL1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DCLK1

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Alternative Name: DCAMKL1 (DCLK1 Products)

Synonyms: DCLK1 antibody, DCAMKL1 antibody, dcamkl1 antibody, dclk1 antibody, wu:fc51f11 antibody, zgc:153709 antibody, MGC145348 antibody, CL1 antibody, CLICK1 antibody, DCDC3A antibody, DCLK antibody, 1700113D08Rik antibody, 2810480F11Rik antibody, AI836758 antibody, Click-I antibody, Cpg16 antibody, Dcamkl1 antibody, Dcl antibody, Dclk antibody, mKIAA0369 antibody, Ania4 antibody, doublecortin like kinase 1 antibody, doublecortin-like kinase 1a antibody, serine/threonine-protein kinase DCLK1 antibody, doublecortin-like kinase 2 antibody, doublecortin-like kinase 1 antibody, DCLK1 antibody, dclk1a antibody, LOC587664 antibody, dclk2 antibody, LOC100539645 antibody, Dclk1 antibody

Background:

Synonyms: Calcium/calmodulin-dependent protein kinase type I-like CPG16, Cpg16, Dcl, Dclk, Dclk1, DCLK1_HUMAN, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1, KIAA0369, Serine/threonine-protein kinase DCAMKL1, Serine/threonine-protein kinase DCLK1.

Background: Lissencephaly (smooth brain) is an abnormality of brain development characterized by incomplete neuronal migration and a smooth cerebral surface, manifesting as severe mental retardation. Genetic analysis has identified two proteins that are mutated in some cases of lissencephaly, designated lissencephaly-1 protein (LIS1) and doublecortin. LIS1 displays sequence homology to ?subunits of heterotrimeric G proteins, and doublecortin contains a consensus Abl phosphorylation site. In addition, the DCAMKL1 (doublecortin-like and CAM kinase-like 1) protein shows homology to doublecortin. All three proteins are highly expressed in developing brain and may function together to regulate microtubules involved in neuronal migration. The DCAMKL1 protein encodes a functional kinase that is capable of phosphorylating myelin basic protein and itself, but its kinase activity does not appear to affect its microtubule polymerization activity.