TSEN15 antibody

Catalog No. ABIN1387164

Product Details anti-TSEN15 Antibody

Target: TSEN15 (tRNA Splicing Endonuclease 15 Homolog (TSEN15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSEN15 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human C1orf19/SEN15

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for TSEN15

Target: TSEN15 (tRNA Splicing Endonuclease 15 Homolog (TSEN15))

Alternative Name: C1orf19 (TSEN15 Products)

Background:

Synonyms: Chromosome 1 open reading frame 19, Chromosome 1 open reading frame 19, isoform CRA_c, HsSen15, sen15, SEN15 homolog, SEN15_HUMAN, tRNA intron endonuclease Sen15, tRNA splicing endonuclease 15 homolog S. cerevisiae, tRNA splicing endonuclease subunit Sen15, tRNA-intron endonuclease SEN15, tRNA-splicing endonuclease subunit SEN15, Tsen15.

Background: SEN15, also known as TSEN15 (tRNA-splicing endonuclease subunit Sen15) or C1orf19, is a nuclear protein that plays a role in tRNA splicing. Widely expressed with highest expression in testis and uterus, SEN15 is a non-catalytic subunit of the multi-protein tRNA-splicing endonuclease complex. The endonuclease complex is responsible for identifying and cleaving pre-tRNA at both 5? and 3? splice sites, thereby releasing introns and free tRNA molecules with 2',3' cyclic phosphates and 5'-OH termini. In addition to its role in pre-tRNA splicing, the endonuclease complex participates in mRNA processing and, via its association with pre-mRNA processing factors, is thought to link pre-tRNA and pre-mRNA splicing events. As a subunit of the complex, SEN15 participates in protein expression and, ultimately, cell growth and division.