C21ORF45 antibody

Catalog No. ABIN1387140

Product Details anti-C21ORF45 Antibody

Target: C21ORF45 (MIS18A) (MIS18 Kinetochore Protein Homolog A (MIS18A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C21ORF45 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FASP1/C21orf45

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for C21ORF45

Target: C21ORF45 (MIS18A) (MIS18 Kinetochore Protein Homolog A (MIS18A))

Alternative Name: FASP1 (MIS18A Products)

Synonyms: B28 antibody, C21orf45 antibody, C21orf46 antibody, FASP1 antibody, MIS18alpha antibody, hMis18alpha antibody, 2610039C10Rik antibody, 2810018N07Rik antibody, RGD1310778 antibody, MIS18 kinetochore protein A antibody, MIS18A antibody, Mis18a antibody

Background:

Synonyms: B28, C21orf46, FASP1, hMis18alpha, MaFF interacting protein, MAFIP, MIS18A, Protein Mis18 alpha, MS18A_HUMAN.

Background: Mapping to chromosome 21, the FASP1 gene (FAPP1-associated protein 1) encodes a 233 amino acid protein that is homologous to the fission yeast protein Mis18. In yeast, Mis18 is localized to the centrosome and forms a complex with Mis16 to maintain the deacetylated state of histones specifically in the central core of centromeres. FASP1, also known as Protein Mis18-alpha and C21orf45, is required for the recruitment of CENP-A to centrosomes and is thereby essential for normal chromosome segregation during mitosis. With expression in testis, FASP1 exists as a homodimer, a heterodimer with MIS18B or is present in a complex containing other Mis18 family members. FASP1 has been shown to bind to pp5644 in Hela cells, where overexpression of pp5644 leads to inhibited growth and colony formation.