DCAF12 antibody

Catalog No. ABIN1387059

Product Details anti-DCAF12 Antibody

Target: DCAF12 (DDB1 and CUL4 Associated Factor 12 (DCAF12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCAF12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DCAF12/WDR40A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DCAF12

Target: DCAF12 (DDB1 and CUL4 Associated Factor 12 (DCAF12))

Alternative Name: DCAF12 (DCAF12 Products)

Background:

Synonyms: cancer/testis antigen 102, Centrosome-related protein TCC52, CT102, DCA12_HUMAN, DCAF12, DDB1- and CUL4-associated factor 12, TCC52, Testis cancer centrosome-related protein, WD repeat domain 40A, WD repeat-containing protein 40A, WDR40A.

Background: WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. WDR40A (WD-repeat-containing protein 40A), also known as DDB1- CUL4-associated factor 12, is a 453 amino acid cytoplasmic protein that contains four WD-repeats. WDR40A is highly expressed in some cancer cell lines, lung cancer tissues and normal testis. A probable substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex, WRD40A is suggested to interact with DDB1. WDR40A is encoded by a gene mapping to human chromosome 9p13.3. Human chromosome 9 houses over 900 genes and comprises nearly 4 % of the human genome.