RBX1 antibody (AA 1-70)

Catalog No. ABIN1386821

Product Details anti-RBX1 Antibody

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Binding Specificity: AA 1-70

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ROC1/RNF75

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RBX1

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Alternative Name: RNF75 (RBX1 Products)

Synonyms: BA554C12.1 antibody, RNF75 antibody, ROC1 antibody, 1500002P15Rik antibody, AA517855 antibody, im:7137515 antibody, zgc:136444 antibody, RBX1 antibody, ATRBX1 antibody, F7C8.160 antibody, F7C8_160 antibody, HRT1 antibody, REGULATOR OF CULLINS-1 antibody, RING-BOX 1 antibody, RING-box 1 antibody, hop21 antibody, BEST:CK01110 antibody, CG16982 antibody, CK01110 antibody, Dmel\CG16982 antibody, EG:115C2.11 antibody, Rbx1 antibody, Rbx1/Roc antibody, Roc antibody, Roc1alpha antibody, anon-EST:Posey65 antibody, dRbx1 antibody, dRoc1 antibody, dRoc1a antibody, ring-box 1 antibody, ring-box 1, E3 ubiquitin protein ligase antibody, RING-box 1 antibody, RING-box protein 1 antibody, RING-box protein 1a antibody, Regulator of cullins 1a antibody, RBX1 antibody, Rbx1 antibody, rbx1 antibody, MGG_08844 antibody, LOC9308017 antibody, rbx-1 antibody, Roc1a antibody

Background:

Synonyms: E3 ubiquitin-protein ligase RBX1, MGC13357, MGC1481, Protein ZYP, Rbx 1, Rbx1, RBX1_HUMAN, Regulator of Cullins 1, Ring box 1, RING box protein 1, RING finger protein 75, RING finger protein, RING-box protein 1, Ringbox protein 1, RNF 75, RNF75, ROC 1, ZYP protein.

Background: E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.

Gene ID: 9978

Pathways: Cell Division Cycle, M Phase, SARS-CoV-2 Protein Interactome