SKP1 antibody (AA 81-163)

Catalog No. ABIN1386819

Product Details anti-SKP1 Antibody

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Binding Specificity: AA 81-163

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SKP1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1 (SKP1 Products)

Background:

Synonyms: Cyclin A/CDK2 associated p19, Cyclin A/CDK2 associated protein p19, Cyclin-A/CDK2-associated protein p19, EMC 19, EMC19, OCP 2, OCP II, OCP II protein, OCP-2, OCP-II, OCP2, OCPII, organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, RNA polymerase II elongation factor like protein, RNA polymerase II elongation factor like protein OCP2, RNA polymerase II elongation factor-like protein, S phase kinase associated protein 1, S phase kinase associated protein 1A, S phase kinase associated protein 1A p19A, S-phase kinase-associated protein 1, SIII, Skp 1, SKP 1A, skp1, SKP1_HUMAN, SKP1A, TCEB1L, Transcription elongation factor B SIII polypeptide 1 like, Transcription Elongation Factor B 1 like, Transcription Elongation Factor B 1-like, Transcription elongation factor B, Transcription elongation factor B SIII polypeptide 1 like.

Background: Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22', the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

Gene ID: 6500

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases