MYF5 antibody (pSer49)

Catalog No. ABIN1386790

Product Details anti-MYF5 Antibody

Target: MYF5 (Myogenic Factor 5 (MYF5))

Binding Specificity: pSer49

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MYF5 around the phosphorylation site of Ser49

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MYF5

Target: MYF5 (Myogenic Factor 5 (MYF5))

Alternative Name: Myf5 (MYF5 Products)

Synonyms: bHLHc2 antibody, B130010J22Rik antibody, Myf-5 antibody, cb641 antibody, myf-5 antibody, zgc:110509 antibody, Xmyf5 antibody, Xmyf-5 antibody, MYF5 antibody, xmyf-5 antibody, MYF-5 antibody, myogenic factor 5 antibody, myogenic factor 5 L homeolog antibody, MYF5 antibody, Myf5 antibody, myf5 antibody, myf5.L antibody

Background:

Synonyms: Myf5 phospho S49, Myf5 phospho Ser49, p-Myf5phospho S49, p-Myf5phospho Ser49, Myf-5 bHLHc2, Class C basic helix loop helix protein 2, Class C basic helix-loop-helix protein 2, Myf 5, Myf-5, Myf5, MYF5_HUMAN, Myogenic factor 5.

Background: Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.

Gene ID: 4617

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development