ABLIM3 antibody (AA 401-500)

Catalog No. ABIN1386608

Product Details anti-ABLIM3 Antibody

Target: ABLIM3 (Actin Binding LIM Protein Family, Member 3 (ABLIM3))

Binding Specificity: AA 401-500

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABLIM3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ABLIM3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ABLIM3

Target: ABLIM3 (Actin Binding LIM Protein Family, Member 3 (ABLIM3))

Alternative Name: ABLIM3 (ABLIM3 Products)

Synonyms: AW990719 antibody, D930036B08Rik antibody, RGD1565118 antibody, actin binding LIM protein family member 3 antibody, actin binding LIM protein family, member 3 antibody, ABLIM3 antibody, Ablim3 antibody

Background:

Synonyms: abLIM-3, Ablim3, ABLM3_HUMAN, Actin binding LIM protein 3, Actin binding LIM protein family member 3, Actin-binding LIM protein 3, Actin-binding LIM protein family member 3, HMFN1661, KIAA0843.

Background: The C. elegans protein Unc-115 mediates axon guidance by modulating the growth cone Actin cytoskeleton in response to signals received by growth cone receptors. The mammalian homolog of Unc-115 is the Actin-binding LIM protein family member 1 (ABLIM1, also designated Limatin). The ABLIM1 protein has an N-terminal domain that contains four double zinc finger motifs, which conform to the LIM motif consensus sequence. ABLIM1 binds to F-Actin through a dematin-like domain and is expressed in retina, brain and muscle tissue. There are four known isoforms of ABLIM1. The gene encoding ABLIM1 maps to a region of chromosome 10 associated with frequent loss of heterozygosity in human tumors, thus identifying ABLIM1 as a candidate tumor suppressor gene. ABLIM2 and ABLIM3 show highest expression in muscle and neuronal tissues, bind to F-Actin, and are localized on stress fibers. They also have been shown to enhance STARS (striated muscle activator of Rho signaling) dependent activation of serum-response factor (SRF), thereby modulating transcription.